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Spectroscopic investigation on the interaction of DNA with superparamagnetic iron oxide nanoparticles doped with chromene via dopamine as cross linker
Maryam Mehdipour 1
Gholamreza Dehghan 1*
Mohammad Ali Hosseinpour Feizi 1
Roghayeh Tarasi 2
Objective(s): The interaction of DNA with iron oxide nanoparticles (SPIONs) was studied to find out the interaction mechanism and design new drug delivery systems.
Materials and Methods: The interaction of calf thymus DNA (ctDNA) with SPIONs doped with 2H-chromene via dopamine as cross linker (SPIONs@DA-Chr) was studied using the UV absorption spectroscopy, viscosity measurement, circular dichroism, fluorescence and FT-IR spectroscopic techniques.
Results: UV absorption study showed hyperchromic effect in the spectra of DNA. Few changes were observed in the viscosity of ctDNA in the presence of different concentration of SPIONs@DA-Chr. The result of circular dichroism (CD) suggested that SPIONs@DA-Chr can change the secondary structure of DNA. Further, fluorescence quenching reaction of ctDNA with SPIONs@DA-Chr and competitive fluorescence spectroscopy studied by using methylene blue, have shown that the SPIONs@DA-Chr can bind to ctDNA through non-intercalative mode. FT-IR spectroscopy confirmed the binding of SPIONs@DA-Chr and ctDNA.
Conclusion: These results suggested that SPIONs@DA-Chr binds to DNA via groove binding mode.
DNA interaction
Dopamine
Iron oxide nanoparticles
Spectroscopy
This work is licensed under the Creative Commons Attribution 4.0 International License.
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1Department of biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
http://creativecommons.org/licenses/by/4.0/.
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