Antonie van Leeuwenhoek, ( ISI ), Volume (104), No (1), Year (2013-4) , Pages (47-54)

Title : ( Marinobacter persicus sp. nov., a moderately halophilic bacterium from a saline lake in Iran )

Authors: Maryam Bagheri , Mohammad Ali Amoozegar , Maryam Didari , Ali Makhdoumi , Peter Schumann , Cathrin Sproer , Cristina Sanchez-Porro , Antonio Ventosa ,

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Abstract

A Gram-negative, non-endospore-forming, rod shaped, strictly aerobic, moderately halophilic bacterium, designated strain M9BT, was isolated from the hypersaline lake Aran-Bidgol in Iran. Cells of strain M9BT were found to be motile and produce colonies with an orange-yellow pigment. Growth was determined to occur between 5 and 20 % (w/v) NaCl and the isolate grew optimally at 7.5-10 % (v/w) NaCl. The optimum pH and temperature for growth of the strain were determined to be pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of 6-8 and 25-45 °C, respectively. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain M9BT is a member of the genus Marinobacter. The closest relative to this strain was found to be Marinobacter hydrocarbonoclasticus MBIC 1303T with a similarity level of 97.7 %. DNA-DNA hybridization between the novel isolate and this phylogenetically related species was 13 ± 2 %. The major cellular fatty acids of the isolate were identified as C16:0, C19:1 ω6c, C18:1 ω9c and C16:1 ω9c. The polar lipid pattern of strain M9BT was determined to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and three phospholipids. Ubiquinone 9 (Q-9) was the only lipoquinone detected. The G+C content of the genomic DNA of this strain was determined to be 58.6 mol%. Phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data suggest that this strain represents a novel species of the genus Marinobacter, for which the name Marinobacter persicus sp. nov. is proposed. The type strain of Marinobacter persicus is strain M9BT (=IBRC-M 10445T = CCM 7970T = CECT 7991T = KCTC 23561T).

Keywords

, Marinobacter persicus,  Hypersaline lake ,  Moderately halophilic bacterium ,  Polyphasic taxonomy
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@article{paperid:1034147,
author = {Maryam Bagheri and Mohammad Ali Amoozegar and Maryam Didari and Makhdoumi, Ali and Peter Schumann and Cathrin Sproer and Cristina Sanchez-Porro and Antonio Ventosa},
title = {Marinobacter persicus sp. nov., a moderately halophilic bacterium from a saline lake in Iran},
journal = {Antonie van Leeuwenhoek},
year = {2013},
volume = {104},
number = {1},
month = {April},
issn = {0003-6072},
pages = {47--54},
numpages = {7},
keywords = {Marinobacter persicus;  Hypersaline lake ; Moderately halophilic bacterium ; Polyphasic taxonomy},
}

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%0 Journal Article
%T Marinobacter persicus sp. nov., a moderately halophilic bacterium from a saline lake in Iran
%A Maryam Bagheri
%A Mohammad Ali Amoozegar
%A Maryam Didari
%A Makhdoumi, Ali
%A Peter Schumann
%A Cathrin Sproer
%A Cristina Sanchez-Porro
%A Antonio Ventosa
%J Antonie van Leeuwenhoek
%@ 0003-6072
%D 2013

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