Turkish Journal of Biology, ( ISI ), Volume (37), No (4), Year (2013-8) , Pages (464-471)

Title : ( Pomegranate (Punica granatum L.) reduces endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in rat )

Authors: Khadijeh Hashemi , Kazem Parivar , - - , Alireza Haghparast , Mehrdad Mohri , Naser Margan Azghadi , M. h. Nazem Shirazi , Amir Afkhami Goli ,

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Ischemia/reperfusion (I/R) is one of the main causes of acute renal failure, leading to generation of reactive oxygen species (ROS) and ensuing oxidative stress, which results in unfolded/misfolded protein accumulation and its dependent response pathways,generally known as unfolded protein response (UPR), in the endoplasmic reticulum (ER). We studied the effects of renal I/R on the expression of ER-stress genes, as well as concomitant effects of oral pretreatment with pomegranate (Punica granatum L.) pith and carpellary membrane (PPCM). An in vivo model of rat kidney I/R followed by conventional and real-time RT-PCR was used to evaluate the modulation of GRP78 and XBP1 as central UPR and ER-stress markers. Ischemia followed by reperfusion (60 and 150 min, respectively) resulted in decreased antioxidant properties of plasma (lowered ferric reducing ability of plasma [FRAP] value) and GRP78 transcript levels. Oral administration of PPCM aqueous extract for 10 days with or without ischemia (PPCM and PPCM/Isc groups, respectively) was able to further decrease the GRP78 expression, while it increased the FRAP value. PPCM pretreatment also reduced the XBP1 splicing in the PPCM/Isc group compared to the Isc group. These results suggest that UPR is activated during oxidative insults induced by I/R, while PPCM can reduce I/R-induced ER stress in rat kidneys via antioxidant defense mechanisms.

Keywords

, Antioxidant, ER stress, GRP78, pomegranate, unfolded protein response,
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@article{paperid:1035633,
author = {Khadijeh Hashemi and Kazem Parivar and -, - and Haghparast, Alireza and Mohri, Mehrdad and Naser Margan Azghadi and M. H. Nazem Shirazi and Afkhami Goli, Amir},
title = {Pomegranate (Punica granatum L.) reduces endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in rat},
journal = {Turkish Journal of Biology},
year = {2013},
volume = {37},
number = {4},
month = {August},
issn = {1300-0152},
pages = {464--471},
numpages = {7},
keywords = {Antioxidant; ER stress; GRP78; pomegranate; unfolded protein response; XBP1},
}

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%0 Journal Article
%T Pomegranate (Punica granatum L.) reduces endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in rat
%A Khadijeh Hashemi
%A Kazem Parivar
%A -, -
%A Haghparast, Alireza
%A Mohri, Mehrdad
%A Naser Margan Azghadi
%A M. H. Nazem Shirazi
%A Afkhami Goli, Amir
%J Turkish Journal of Biology
%@ 1300-0152
%D 2013

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