Dog rose (Rosa canina) is one of the most important ornamental and medicinal plants which is used as rootstock for ornamental roses as Rosa hybrid and Rosa floribunda. In vitro propagation of rose has played a very important role in rapid multiplication of species with desirable traits and production of healthy and disease-free plants. Micropropagation, using nodal segments of Rosa canina under different combinations of BAP (0, 0.5, 1, 1.5, 2 Mgl ⁻ 1 ), GA 3 (0, 0.5 Mgl ⁻ 1 ) and NAA (0, 0.5 Mgl ⁻ 1 ) on MS and VS medium in proliferation stage and different combinations of NAA and IBA (0, 0.3, 0.6, 0.9 Mgl ⁻ 1 ) on VS and ½ VS medium in rooting stage was revised. The results showed that the highest shoot proliferation was obtained on VS medium containing 2 Mgl ⁻ 1 BAP. Furthermore, in rooting stage the highest root induction obtained in half strength VS full medium containing 0.6 – 0.9 Mgl ⁻ 1 each of NAA or IBA. The present investigation recommended a practicable in vitro plant protocol for R.canina as an important step for successful implementation of biotechnological techniques for rose improvement.

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