Journal of Biological Chemistry, Volume (284), No (17), Year (2009-4) , Pages (11070-11079)

Title : ( The Fission Yeast SEL1 Domain Protein Cfh3p A NOVEL REGULATOR OF THE GLUCAN SYNTHASE Bgs1p WHOSE FUNCTION IS MORE RELEVANT UNDER STRESS CONDITIONS )

Authors: Mirza Mohammad Reza Sharifmoghadam , M.-Henar Valdivieso ,

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Abstract

In Schizosaccharomyces pombe, Bgs1/Cps1p is a (1,3)-glucan synthase required for linear(1,3)-glucan synthesis and primary septum formation. Here, we have studied the regulation of Bgs1p by Cfh3/Chr4p, a member of a family of conserved adaptor proteins, which resembles the chitin synthase regulator Chs4p from Saccharomyces cerevisiae and Candida albicans. cfh3 cells showed a genetic interaction with cps1-191, and Cfh3p co-immunoprecipitated with Bgs1/Cps1p. In the absence of cfh3, cells were more sensitive to digestion by glucanases, and both Calcofluor staining and glucan synthesis were reduced. We found that in a wild-type strain,(1,3)-glucan synthesis was reduced under stress conditions. In the cfh3, cps1-191, and cfh3 cps1-191 strains, (1,3)-glucan synthesis was further reduced, and growth was impaired under stress conditions, suggesting that Cfh3p and Bgs1p might play a role in ensuring growth in unfavorable environments. In a cfh3 mutant, Bgs1p was delocalized when the cells were distressed, but a blockade in endocytosis prevented this delocalization. Finally, we found that the SEL1 repeats are required for Cfh3p function. These results show that Cfh3p is a regulatory protein for Bgs1p and that its function is particularly necessary when the cells are undergoing stress.

Keywords

, Schizosaccharomyces pombe, Bgs1/Cps1p, Cfh3p, Yeast, SEL1 Domain Protein,
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@article{paperid:1045786,
author = {Sharifmoghadam, Mirza Mohammad Reza and M.-Henar Valdivieso},
title = {The Fission Yeast SEL1 Domain Protein Cfh3p A NOVEL REGULATOR OF THE GLUCAN SYNTHASE Bgs1p WHOSE FUNCTION IS MORE RELEVANT UNDER STRESS CONDITIONS},
journal = {Journal of Biological Chemistry},
year = {2009},
volume = {284},
number = {17},
month = {April},
issn = {0021-9258},
pages = {11070--11079},
numpages = {9},
keywords = {Schizosaccharomyces pombe; Bgs1/Cps1p; Cfh3p; Yeast; SEL1 Domain Protein;},
}

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%0 Journal Article
%T The Fission Yeast SEL1 Domain Protein Cfh3p A NOVEL REGULATOR OF THE GLUCAN SYNTHASE Bgs1p WHOSE FUNCTION IS MORE RELEVANT UNDER STRESS CONDITIONS
%A Sharifmoghadam, Mirza Mohammad Reza
%A M.-Henar Valdivieso
%J Journal of Biological Chemistry
%@ 0021-9258
%D 2009

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