International Journal of Pharmaceutics, Volume (515), No (2), Year (2016-12) , Pages (708-720)

Title : ( Induction of a balanced Th1/Th2 immune responses by co-delivery of PLGA/ovalbumin nanospheres and CpG ODNs/PEI-SWCNT nanoparticles as TLR9 agonist in BALB/c mice )

Authors: Mahboubeh Ebrahimian , Maryam Hashemi , MOHSEN MALEKI , Khalil Abnous , GHOLAMREZA HASHEMI TABAR , Mohammad Ramezani , Alireza Haghparast ,

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Abstract

To develop effective and safe vaccines with reduced dose of antigen and adjuvant, intelligent delivery systems are required. Many delivery systems have been developed to enhance the biological activity of cytosine-phosphorothioate-guanine oligodeoxynucleotides (CpG ODN) as both immunotherapeutic agents and vaccine adjuvants. In this study we designed a novel CpG ODN delivery system based on single-walled carbon nanotube (SWCNT) functionalized with polyethylenimine (PEI) and alkylcarboxylated PEI (AL-PEI). The physicochemical characteristics, cytotoxicity and cellular uptake studies of these carriers were performed. All carriers were conjugated with CpG ODN followed by co-delivery with ovalbumin (OVA) encapsulated into poly (lactic-co-glycolic acid) nanospheres (PLGA NSs) to enhance the induction of immune responses. The effect of these formulations on antibody (IgG1, IgG2a) and cytokine (IL-1β, IFN-γ, IL-4) production was evaluated in an in vivo experiment. The results showed that all nano-adjuvant formulations had a strong influence in up-regulation of IFN-γ and IL-4 in parallel with high IgG1-IgG2a isotype antibody titers in mice. In particular, SWCNT-AL-PEI nano-adjuvant formulation generated a balanced Th1 and Th2 immune response with more biased toward Th1 response without exhibiting any inflammatory and toxic effects. Therefore this nano-adjuvant formulation could be used as an efficient prophylactic immune responses agent.

Keywords

, Single, walled carbon nanotubes; Polyethylenimine; CpG ODN; Adjuvant delivery; PLGA; Ovalbumin
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@article{paperid:1059465,
author = {Ebrahimian, Mahboubeh and Maryam Hashemi and MALEKI, MOHSEN and Khalil Abnous and HASHEMI TABAR, GHOLAMREZA and Mohammad Ramezani and Haghparast, Alireza},
title = {Induction of a balanced Th1/Th2 immune responses by co-delivery of PLGA/ovalbumin nanospheres and CpG ODNs/PEI-SWCNT nanoparticles as TLR9 agonist in BALB/c mice},
journal = {International Journal of Pharmaceutics},
year = {2016},
volume = {515},
number = {2},
month = {December},
issn = {0378-5173},
pages = {708--720},
numpages = {12},
keywords = {Single-walled carbon nanotubes; Polyethylenimine; CpG ODN; Adjuvant delivery; PLGA; Ovalbumin},
}

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%0 Journal Article
%T Induction of a balanced Th1/Th2 immune responses by co-delivery of PLGA/ovalbumin nanospheres and CpG ODNs/PEI-SWCNT nanoparticles as TLR9 agonist in BALB/c mice
%A Ebrahimian, Mahboubeh
%A Maryam Hashemi
%A MALEKI, MOHSEN
%A Khalil Abnous
%A HASHEMI TABAR, GHOLAMREZA
%A Mohammad Ramezani
%A Haghparast, Alireza
%J International Journal of Pharmaceutics
%@ 0378-5173
%D 2016

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