@article{paperid:1027480,
author = {Alfred King-Yin Lam and Vinod Gopalan and Nassiri, Mohammadreza and Kais Kasim and Jayampathy Dissanayake and Johnny Chuek-On Tang and Robert Anthony Smith},
title = {Altered JS‐2 expression in colorectal cancers and its clinical pathological relevance},
journal = {Molecular Oncology},
year = {2011},
volume = {5},
number = {5},
month = {October},
issn = {1574-7891},
pages = {475--481},
			numpages = {6},
keywords = {JS-2 is a novel gene located at 5p15.2 and originally detected in primary oesophageal cancer.
There is no study on the role of JS-2 in colorectal cancer. The aim of this study is to determine
the gene copy number and expression of JS-2 in a large cohort of patients with colorectal
tumours and correlate these to the clinicopathological features of the cancer patients.We
evaluated the DNA copy number and mRNA expression of JS-2 in 176 colorectal tissues (116
adenocarcinomas; 30 adenomas and 30 non-neoplastic tissues) using real-time polymerase
chain reaction. JS-2 expression was also evaluated in two colorectal cancer cell lines and a benign
colorectal cell line. JS-2 amplification was noted in 35% of the colorectal adenocarcinomas.
Significant differences in relative expression levels for JS-2 mRNA between
different colorectal tissues were noted ( p ¼ 0.05). Distal colorectal adenocarcinoma had significantly
higher copy number than proximal adenocarcinoma ( p ¼ 0.005). The relative expression
level of JS-2 was different between colonic and rectal adenocarcinoma ( p ¼ 0.007).
Mucinous adenocarcinoma showed higher JS-2 expression than non-mucinous adenocarcinoma
( p ¼ 0.02). Early T-stage cancers appear to have higher JS-2 copy number and lower
expression of JS-2 mRNA than later stage cancers ( p ¼ 0.001 and 0.03 respectively). Colorectal
cancer cell lines showed lower expression of JS-2 than the benign colorectal cell line. JS-2
copy number change and expression were shown for the first time to be altered in the carcinogenesis
of colorectal cancer. In addition; genetic alteration of JS-2 was found to be related
to location; pathological subtypes and staging of colorectal cancer},
}