@inproceedings{paperid:1046081,
author = {Haghparast, Alireza and Soleymani, Nooshinmehr and Borji, Hassan and Naghibi, Abolghasem and Taran, Fatemeh and محمد حسین ناظم شیرازی and مریم ترابی},
title = {Differential expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells cultured with hydatid cyst derived antigens},
booktitle = {15th International Congress of Immunology},
year = {2013},
location = {میلان, ITALY},
keywords = {chain reaction (qRT-PCR). Somatic and exceretory/secretory antigens of protoscoleces (PSC); germainal layer (GL) and hydatid cyst fluid (HCF) antigens were prepared to stimulate ovine PBMCs. A higher expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of PSC; whereas only TLR2 was up-regulated by ES antigens of PSC. Similarly; we detected an increased level of TLR2 and TLR4 expression in PBMCs cultured with GL antigens. In contrast; the expression of TLR2 and TLR4 in PBMCs was down-regulated by HCF antigens. The results presented in this study suggest that; PSC (Somatic and excretory/secretory antigen) and GL antigens have immunostimulatory effects whereas HCF antigens exhibit an immunoregulatory effect on peripheral immune cells. In conclusion; this study provide for the first time evidence of induction of TLRs in ovine PBMCs by PSC; GL and HCF antigens of Echinococcus.
P4.07.15},
}