@article{paperid:1088254,
author = {Gonbadi, Parisa and Jalal, Razieh and Akhlaghinia, Batool and Ghasemzadeh, Maryam Sadat},
title = {Tannic acid-modified magnetic hydrotalcite-based MgAl nanoparticles for the in vitro targeted delivery of doxorubicin to the estrogen receptor-overexpressing colorectal cancer cells},
journal = {},
year = {2022},
volume = {68},
month = {February},
pages = {103026--103034},
			numpages = {8},
keywords = {In the present study; the tannic acid-modified magnetic hydrotalcite-based MgAl nanoparticles (TA@HT@Fe3O4
NPs) were synthesized and assessed as a novel magnetic-targeted drug delivery system to the estrogen receptor
(ER)-expressing colorectal cancer cells in vitro. In addition; FT-IR; EDS; DLS; and XRD measurements were
employed for confirming the successful loading of doxorubicin (DOX); a widely-used chemotherapeutic agent; on
the TA@HT@Fe3O4 NPs. The NPs exhibited a negative zeta potential value due to the presence of negatively
charged molecules on their surface. The lading of DOX on the TA@HT@Fe3O4 NPs resulted in reducing their
negative charge. TEM and FESEM images exhibited a layered HT structure with irregular edges and a mean size
of 70 nm Fe3O4 NPs were visualized as small quasi-spherical particles with a uniform size of 12 nm. The
TA@HT@Fe3O4 NPs had higher solubility at acidic pH than that at pH 7.4. Further; the entrapment efficiency of
DOX in the TA@HT@Fe3O4 NPs was obtained about 51% at pH 7.4. The in vitro release of the DOX/
TA@HT@Fe3O4 NPs indicated a pH-dependent biphasic and sustained release of DOX as an initial rapid release
in the first 24 h; followed by a 120-h sustained release. Furthermore; the safety and biocompatibility of the
TA@HT@Fe3O4 NPs were confirmed through using hemolysis and MTT assays. Based on the results of fluorescence
microscopy and flow cytometry; a higher DOX cellular uptake was observed in the ER-positive HCT116
and LoVo cells treated with the DOX/TA@HT@Fe3O4 NPs compared to the ER-negative HEK293 ones. Treatment
with the DOX/TA@HT@Fe3O4 NPs led to more cytotoxicity than free DOX did and enhanced the DOX-induced
reactive oxygen species (ROS) generation and subsequent apoptosis in colorectal cancer cells. Finally; applying a
0.6 T static magnetic field resulted in improving the anti-proliferative activity of the DOX/TA@HT@Fe3O4 NPs
in HCT116 cells in a time exposure-dependent manner and enhancing ROS production. In general; the prepared
NPs can serve as a promising candidate for the pH-responsive and magnetic-targeted delivery to the ERexpressing
cells.},
}