@article{paperid:1097283,
author = {محمد ثابتی and محمد سقیب احسان and دینا آدمی and سیده نفیسه حسنی and سیاوش موشخیان and ریحانه شفیعیان and Salari Sedigh, Hamideh and جمیله قدوسی},
title = {Cell-Based Regenerative Endodontics for the Treatment of Irreversible Pulpitis: An In Vivo Investigation},
journal = {Journal of Endodontics},
year = {2023},
volume = {50},
number = {3},
month = {December},
issn = {0099-2399},
pages = {344--350},
			numpages = {6},
keywords = {Introduction
This study aims to investigate the ability of umbilical cord mesenchymal stem cells (UC-MSC) to enhance the regeneration of pulp-dentin complex in immature permanent teeth with irreversible pulpitis.
Methods
A total of 32 mandibular premolar teeth with immature apices in 5 dogs were used in this in-vivo randomized controlled trial. Eight healthy teeth without pre-existing pathosis served as the positive control samples and received no treatment; while in another eight teeth; the pulp was completely extirpated (negative control). Class V cavities were prepared to induce inflammation in the remaining 16 teeth (groups 3 and 4) and the pulp was extirpated 2-4 mm short of the radiographic apex. Of the 16; the eight teeth in group 4 received 1 mL of cord blood stem cells with a hydrogel scaffold. Blood clots were covered with mineral trioxide aggregates at the cementoenamel junction in the experimental groups; and teeth were filled with RMGI and composite. Three months later; block sections were removed for histologic evaluations for the evaluation of post-operative apical closure; degree of inflammation; and presence of normal pulp tissue. The data were statistically analyzed with the chi-square test (P < .05).
Results
All teeth with complete pulp extirpation demonstrated pulpal necrosis with no post-operative closure of their apices; while apical closure was seen in all the teeth in the remaining groups. There was a statistically significant (P <0.001) difference in the presence of inflammation and normal pulp tissue between the experimental groups. The teeth in group 3 showed normal pulp tissue extending to the level of MTA; but there was inflammation within the canal space. In contrast; the teeth in the UC-MSC group demonstrated organized; normal pulp tissue with no inflammation.
Conclusion
Based on these results; the regeneration of the pulp-dentin complex is possible with no inflammation when UC-MSCs are used and 2-4 mm of the apical pulp remains intact in immature teeth with irreversible pulpitis.},
}