Russian Journal of Genetics, ( ISI ), Volume (41), No (6), Year (2005-3) , Pages (817-822)

Title : Analysis and Frequency of Bovine Lymphocyte Antigen (BoLA-DRB3) Alleles in Iranian Holstein Cattle ( Analysis and Frequency of Bovine Lymphocyte Antigen (BoLA-DRB3) Alleles in Iranian Holstein Cattle )

Authors: Mohammadreza Nassiri , Feradoun EFTEKHARI SHAHROUDI , Jafar Mosafer , - - , , , M. R. Mohammad Abadi , G. E. Sulimova ,

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Abstract

Abstract The bovine lymphocyte antigen (BoLA-DRB3) gene encodes cell surface glycoproteins that initiate immune response by presenting processed antigenic peptides to CD4 T helper cells. DRB3 is the most polymorphic bovine MHC class II gene which encodes the peptide-binding groove. DRB3 gene has been extensively evaluated as a candidate marker for association with various bovine diseases and immunological traits. This study describes genetic variability in the BoLA-DRB3 in Iranian Holstein cattle. This is the first study of the DNA polymorphism of the BoLA-DRB3 gene in Iranian Holstein cattle. Hemi-nested PCR-RFLP method is used for identification the frequency of BoLA-DRB3 alleles. The BoLA-DRB3 locus is highly polymorphic in the studied herd (26 alleles). Almost 67% of the alleles were accounted for four alleles (BoLA-DRB3.2*8, *24, *11, and *16) in Iranian Holstein cattle. The DRB3.2*8 allele frequency (26.6%) was higher than the others. The frequencies of the DRB3.2*54, *37, *36, *28, *25, *14, *13, *10, *1 alleles were lower than 1%. Significant distinctions have been found between Iranian Holstein cattle and other cattle breeds studied. In Iranian Holstein cattle the alleles (BoLA-DRB3.2*22, *2, and *16) associated with a lower risk of cystic ovarian disease in Holstein cattle are found. The alleles associated with the resistance to mastitis and to bovine leukemia virus infection BoLA-DRB3.2*11 and *23 are detected with the frequencies 10.4 and 4.4%, respectively. Thus, in the Iranian Holstein cows studied alleles associated with resistance to various diseases are found. The method of DNA-typing of animals can be used in agricultural practice for BoLA-DRB3 allele genotyping of cattle in order to reduce spreading of alleles providing susceptibility to mastitis or leukemia in cattle herds

Keywords

, BoLA-DRB3, Iranian Holstein, Frequency
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@article{paperid:201929,
author = {Nassiri, Mohammadreza and EFTEKHARI SHAHROUDI, Feradoun and Mosafer, Jafar and -, - and , and , and M. R. Mohammad Abadi and G. E. Sulimova},
title = {Analysis and Frequency of Bovine Lymphocyte Antigen (BoLA-DRB3) Alleles in Iranian Holstein Cattle},
journal = {Russian Journal of Genetics},
year = {2005},
volume = {41},
number = {6},
month = {March},
issn = {1022-7954},
pages = {817--822},
numpages = {5},
keywords = {BoLA-DRB3; Iranian Holstein; Frequency},
}

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%0 Journal Article
%T Analysis and Frequency of Bovine Lymphocyte Antigen (BoLA-DRB3) Alleles in Iranian Holstein Cattle
%A Nassiri, Mohammadreza
%A EFTEKHARI SHAHROUDI, Feradoun
%A Mosafer, Jafar
%A -, -
%A ,
%A ,
%A M. R. Mohammad Abadi
%A G. E. Sulimova
%J Russian Journal of Genetics
%@ 1022-7954
%D 2005

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