Summary: The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, during the autumn months of 2005, total of 100 samples from poultry carcasses, representing 20 broiler flocks were obtained by rinse test, after the chilling stage of processing and enriched in Preston broth, followed by streaking on selective media, the suspected colonies were isolated on sheep blood agar and tested for morphology, motility and gram staining. Biochemical tests and hippurate hydrolysis activity were also performed. Concurrently, a multiplex PCR assay (mPCR) with two sets of primers was employed for identification of Campylobacter genus and campylobacter coli. The m-PCR assay was applied on bacterial cultures harvested from selective media plates. By conventional culture method including hippurate hydrolysis test from suspected colonies, 76% of samples were determined as Campylobacter spp., and 2% as campylobacter coli. In m-PCR assay 28% of cultures harvest, identified as Campylobacter genus but Campylobacter coli were not detected in any of the samples. According to this preliminary study, it seems that the contamination rate of poultry carcasses with other species of Campylobacter genus is higher than contamination with Campylobacter coli.

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