Title : ( Detection of Mycobacterium paratuberculosis in Feces and Milk Samples from Holstein Dairy Cows by PCR )
Authors: - - , Mohammadreza Nassiri , Feradoun EFTEKHARI SHAHROUDI , Mohammad Reza Bassami ,
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Abstract
Mycobacterium paratuberculosis, an acid-fast bacterium is the agent of Johne`s disease, an intestinal disease that cause poor nutrient intake in ruminants. Because of the heavy economic loses due to this disease, a diagnostic procedure must be defined that could determine the disease in its initial stages. PCR method based on specific IS900 locus primers was used. Feces and milk samples were collected from 68 Holstein dairy cows. 19 feces samples (of 68 samples) were PCR positive whereas 10 milk samples (of 56 samples) were positive. Results of this study showed that PCR might be the preferred method for detection of the disease with feces and milk samples because of its simplicity, sensitivity and rapidity.
Keywords
Johne`s disease ; Mycobacterium paratuberculosis ; PCR ; Holstein dairy cows
@article{paperid:1008347,
author = { -, - and Nassiri, Mohammadreza and EFTEKHARI SHAHROUDI, Feradoun and Bassami, Mohammad Reza},
title = {Detection of Mycobacterium paratuberculosis in Feces and Milk Samples from Holstein Dairy Cows by PCR},
journal = {Biotechnology},
year = {2008},
volume = {7},
number = {3},
month = {June},
issn = {1682-296X},
pages = {582--585},
numpages = {3},
keywords = {Johne`s disease ; Mycobacterium paratuberculosis ; PCR ; Holstein dairy cows},
}
%0 Journal Article
%T Detection of Mycobacterium paratuberculosis in Feces and Milk Samples from Holstein Dairy Cows by PCR
%A -, -
%A Nassiri, Mohammadreza
%A EFTEKHARI SHAHROUDI, Feradoun
%A Bassami, Mohammad Reza
%J Biotechnology
%@ 1682-296X
%D 2008
