Peganum harmala L. (Zygophyllaceae) is a herb native to dry area .Total alkaloid content of P. harmala varied between 2 and 5% . The alkaloids harmol, harmine and harmaline in P. harmala extracts have a wide spectrum of pharmacological actions.Alkaloids are inhibitors of photophosphorylation in spinach chloroplasts(1). Two research works were conducted with plant above ground biomass and root of Peganum harmala, each with five extract concentrations of 0, 1, 3, 5 % (V/V) in laboratory, and in greenhouse condition five plant material levels of 0, 2, 5, 10 and 20% (W/W) of the above two treatments tested on germination and growth of Anthemis nobilis L.In laboratory ,root of P.harmala had more negative impact on seed germination, radicle and plumule lengths of A. nobilis than the above ground biomass.Germination percentage, radicle and plumule lengths were reduced with increasing extract concentration. Root extract with 3% concentration imposed more reduction in germination percentage than above ground biomass extract with similar concentration (2,3,4).Root of P.harmala imposed maximum reductions in plumule and radicle lengths and their ratio compared to the above ground biomass.

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