Bioprocess and Biosystems Engineering, ( ISI ), Year (2007-11)

Title : ( Metabolic Flux Analysis for Optimizing the Specific Growth Rate of Recombinant Aspergillus niger )

Authors: REZA GHeshlaghi , Jeno Scharer , Murray Moo-Young , Peter Douglas ,

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A comprehensive metabolic network comprising three intracellular compartments (cytoplasm, mitochondrion and peroxisome) was developed for Aspergillus niger. The metabolic flux network includes carbohydrate and amino acid metabolism in both anabolic and catabolic reactions. Linear programming was used for the optimization of the specific growth rates in combination with 37 measured input and output fluxes of the key metabolites to evaluate corresponding intracellular flux distributions throughout the batch fermentations. Logarithmic sensitivity analysis revealed that the addition of proline, alanine and glutamate benefited growth in defined media. The experimental observations and flux analysis showed that tyrosine was a potential candidate for biomass production improvement. Model predictions was verified by conducting batch and fed-batch fermentations and it was found that the addition of the four amino acids according to the predetermined schedule resulted in a 44% and 41% improvements in biomass and recombinant protein productions, respectively.

Keywords

, Aspergillus niger, hen egg white lysozyme, metabolic flux analysis, logarithmic sensitivity, metabolic
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@article{paperid:1010461,
author = {GHeshlaghi, REZA and Jeno Scharer and Murray Moo-Young and Peter Douglas},
title = {Metabolic Flux Analysis for Optimizing the Specific Growth Rate of Recombinant Aspergillus niger},
journal = {Bioprocess and Biosystems Engineering},
year = {2007},
month = {November},
issn = {1615-7591},
keywords = {Aspergillus niger; hen egg white lysozyme; metabolic flux analysis; logarithmic sensitivity; metabolic pathways},
}

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%0 Journal Article
%T Metabolic Flux Analysis for Optimizing the Specific Growth Rate of Recombinant Aspergillus niger
%A GHeshlaghi, REZA
%A Jeno Scharer
%A Murray Moo-Young
%A Peter Douglas
%J Bioprocess and Biosystems Engineering
%@ 1615-7591
%D 2007

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