Title : ( Development and Use of Quantitative Competitive PCR Assay for Detection of Poultry DNA in Sausage )
Authors: Ali Asghar Aslaminejad , Mohammadreza Nassiri , , Morteza Mahdavi , Mohammad Hadi Sekhavati , Ali Javadmanesh ,
Full TextAbstract
This study focused on the development and evaluation of a quantitative competitive polymerase chain reaction (QC-PCR) for detection and quantification of poultry DNA in sausage. PCR is well known to be quantitative if internal DNA standards are coamplified together with the target DNA. A DNA competitor differing by 83 bp in length from the poultry target sequence was constructed and used for PCR together with the target DNA. Specificity of the new primers was evaluated with DNA from cattle and sheep. The results of QC-PCR showed that the percentage of contamination was in the range of 23.87–52.06%.
Keywords
contamination; poultry DNA; quantitative competitive PCR; sausage
@article{paperid:1017326,
author = {Aslaminejad, Ali Asghar and Nassiri, Mohammadreza and , and Mahdavi, Morteza and Sekhavati, Mohammad Hadi and Javadmanesh, Ali},
title = {Development and Use of Quantitative Competitive PCR Assay for Detection of Poultry DNA in Sausage},
journal = {Food Biotechnology},
year = {2010},
volume = {24},
number = {3},
month = {August},
issn = {0890-5436},
pages = {248--257},
numpages = {9},
keywords = {contamination; poultry DNA; quantitative competitive PCR; sausage},
}
%0 Journal Article
%T Development and Use of Quantitative Competitive PCR Assay for Detection of Poultry DNA in Sausage
%A Aslaminejad, Ali Asghar
%A Nassiri, Mohammadreza
%A ,
%A Mahdavi, Morteza
%A Sekhavati, Mohammad Hadi
%A Javadmanesh, Ali
%J Food Biotechnology
%@ 0890-5436
%D 2010
