The effect of 6-amino-5-cyano-3-methyl-1,4-diphenyl-1,4-dihydropyrano[2,3-c]pyrazole as a chelating agent on the excretion of cadmium was evaluated in cadmium-poisoned Wistar rats following food and drink administration. The above chelating agent was prepared in a one step procedure from a three-component reaction of 3-alkyl-1-phenyl-2-pyrazolin-5-one, aryl aldehydes and malononitrile in the presence of base catalysts such as sodium acetate and magnesium oxide (MgO). High surface area MgO as a highly effective heterogeneous base catalyst effectively catalyzes these reactions. The present research aimed to characterize the potential efficiency of our presented chelator as an oral drug chelator after cadmium administration for 60 days. This chelator significantly enhanced the urinary and biliary excretion of cadmium and restored the altered levels of iron. The chelator was given orally to different groups of rats for a period of one week immediately after cadmium administration. After chelation therapy, animals were killed by exsanguinations from abdominal aorta. Blood, kidneys, liver and heart samples were collected and prepared for determination of cadmium. Cadmium and iron concentrations in various tissues were determined by graphite furnace and flame atomic absorption spectrometry (GFAAS and FAAS) methods, respectively. The chelation therapy results showed the present chelator is able to remove cadmium ions from different tissues while iron concentration return to normal level and clinical symptoms also reduce.

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