Title : ( Immobilization of α-amylase onto magnetic nanoparticles by shaking method )
Authors: Razieh Jalal , Zeinab Mortazavi , Elaheh Kafshdare Goharshadi ,
Full TextAbstract
α-amylase was immobilized covalently on Fe3O4 magnetic nanoparticles via carbodiimide activation. The efficiency of immobilization and residual activity of the immobilized α-amylase was dependent upon the immobilization temperature and mass ratio of nanoparticles: carbodiimide: α-amylase. Confirmation of the binding was demonstrated by Fourier transform infrared (FTIR) spectroscopy. At room temperature, immobilized amylase displayed more binding efficiency and less residual activity compared with of 4°C, hence 4°C was chosen as optimum immobilization temperature. The 4:1:1 ratio displayed maximum residual activity therefore, it was chosen as a best ratio for immobilization. The optimum pH value for both free and immobilized amylase was at pH 5 but immobilized amylase exhibited more pH sensitivity at the pH values 6, 7 compared with free amylase and there were no differences in pH profiles for amylase that immobilized in room temperature or 4°C.
Keywords
, α-amylase, Magnetic nanoparticles, Immobilization, Carbodiimide
@inproceedings{paperid:1024718,
author = {Jalal, Razieh and Mortazavi, Zeinab and Kafshdare Goharshadi, Elaheh},
title = {Immobilization of α-amylase onto magnetic nanoparticles by shaking method},
booktitle = {اولین کنگره ملی شیمی نوین ایران},
year = {2011},
location = {شیراز, IRAN},
keywords = {α-amylase; Magnetic nanoparticles; Immobilization; Carbodiimide},
}
%0 Conference Proceedings
%T Immobilization of α-amylase onto magnetic nanoparticles by shaking method
%A Jalal, Razieh
%A Mortazavi, Zeinab
%A Kafshdare Goharshadi, Elaheh
%J اولین کنگره ملی شیمی نوین ایران
%D 2011
