Microbes and Environments, ( ISI ), Volume (27), No (1), Year (2012-1) , Pages (87-93)

Title : ( Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran )

Authors: Ali Makhdoumi , M. A. Amoozegar , B. Kazemi , L. Pasic , A. Ventosa ,

Citation: BibTeX | EndNote

Abstract

Prokaryotic diversity in Aran-Bidgol salt lake, a thalasohaline lake in Iran, was studied by fluorescence in situ hybridization (FISH), cultivation techniques, denaturing gradient gel electrophoresis (DGGE) of PCR-amplified fragments of 16S rRNA genes and 16S rRNA gene clone library analysis. Viable counts obtained (2.5-4 × 106 cells ml-1) were similar to total cell abundances in the lake determined by DAPI direct count (3-4×107 cells ml-1). The proportion of Bacteria to Archaea in the community detectable by FISH was unexpectedly high and ranged between 1:3 and 1:2. We analyzed 101 archaeal isolates and found that most of them belong to the genera Halorubrum (55%) and Haloarcula (18%). Eleven bacterial isolates obtained in pure culture were affiliated with the genera Salinibacter (18.7%), Salicola (18.7%) and Rhodovibrio (35.3%). Analysis of inserts of 100 clones from the eight 16S rRNA clone libraries constructed revealed a total of 37 OTUs. A majority (63%) of these sequences were not related to any previously identified taxa. Within this sampling effort we most frequently retrieved phylotypes related to Halorhabdus (16% of archaeal sequences obtained) and Salinibacter (36% of bacterial sequences obtained). Other prokaryotic groups that were abundant included representatives of Haloquadratum, the anaerobic genera Halanaerobium and Halocella, purple sulfur bacteria of the genus Halorhodospira and Cyanobacteria.

Keywords

, Archaea, Bacteria, Halophilic microorganisms, Prokaryotic diversity, Hypersaline lake
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@article{paperid:1027638,
author = {Makhdoumi, Ali and M. A. Amoozegar and B. Kazemi and L. Pasic and A. Ventosa},
title = {Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran},
journal = {Microbes and Environments},
year = {2012},
volume = {27},
number = {1},
month = {January},
issn = {1342-6311},
pages = {87--93},
numpages = {6},
keywords = {Archaea; Bacteria; Halophilic microorganisms; Prokaryotic diversity; Hypersaline lake},
}

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%0 Journal Article
%T Prokaryotic Diversity in Aran-Bidgol Salt Lake, the Largest Hypersaline Playa in Iran
%A Makhdoumi, Ali
%A M. A. Amoozegar
%A B. Kazemi
%A L. Pasic
%A A. Ventosa
%J Microbes and Environments
%@ 1342-6311
%D 2012

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