Title : ( Use of fluorescent DNA-intercalating dyes in the analysis of DNA via ion-pair reversed-phase denaturing high-performance liquid chromatography )
Authors: Ahmad Reza Bahrami , MarkJ. Dickman , Maryam Moghaddam Matin , John R. Ashby , Paul E. Brown , Matthew J. Conroy , Gregory J.S. Fowler , James P. Rose , Qaiser I. Sheikh , Anthony T. Yeung , David P. Hornby ,
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Abstract
SYBR Green 1 is an asymmetrical cyanine DNA-binding dye that provides an opportunity for increasing the sensitivity of nucleic acid detection when used in conjunction with gel electrophoresis. In this paper, we summarize the general properties and specific uses of SYBR green 1 in ion-pair reversed-phase denaturing high-performance liquid chromatography (IP DHPLC). We describe several applications for the WAVE DHPLC platform that illustrate the generic potential of such intercalating dyes in mutation detection and gene expression profiling. We show that SYBR Green 1 obviates the need to use end-labeled oligodeoxynucleotides for the sensitive detection of nucleic acids during chromatography. Moreover the incorporation of SYBR Green 1 into samples and elution buffers does not impair resolution and has no significant effect on the retention times of DNA fragments compared with dye-free DHPLC
Keywords
, SYBR Green, WAVE DHPLC, DNA-binding dye, liquid chromatography.
@article{paperid:1029861,
author = {Bahrami, Ahmad Reza and MarkJ. Dickman and Moghaddam Matin, Maryam and John R. Ashby and Paul E. Brown and Matthew J. Conroy and Gregory J.S. Fowler and James P. Rose and Qaiser I. Sheikh and Anthony T. Yeung and David P. Hornby},
title = {Use of fluorescent DNA-intercalating dyes in the analysis of DNA via ion-pair reversed-phase denaturing high-performance liquid chromatography},
journal = {Analytical Biochemistry},
year = {2002},
volume = {309},
number = {2},
month = {October},
issn = {0003-2697},
pages = {248--252},
numpages = {4},
keywords = {SYBR Green; WAVE DHPLC;DNA-binding dye; liquid chromatography.},
}
%0 Journal Article
%T Use of fluorescent DNA-intercalating dyes in the analysis of DNA via ion-pair reversed-phase denaturing high-performance liquid chromatography
%A Bahrami, Ahmad Reza
%A MarkJ. Dickman
%A Moghaddam Matin, Maryam
%A John R. Ashby
%A Paul E. Brown
%A Matthew J. Conroy
%A Gregory J.S. Fowler
%A James P. Rose
%A Qaiser I. Sheikh
%A Anthony T. Yeung
%A David P. Hornby
%J Analytical Biochemistry
%@ 0003-2697
%D 2002
