Many feed and forages are exposed to low, medium or high levels of organophosphorous (OP) pesticides. It is unclear whether these exposures (for example diazinon as an OP pesticide) impact dry matter degradability parameters. So, different levels (0, 0.7, 2.8 and 5.6 mg) of diazinon as an OP pesticide with different levels of calcium bentonite (CB) (0 and 100 mg) as a toxin binder were tested for their toxicological effects on in vitro dry matter disappearance. Also we investigated whether CB can inactivate the probably deleterious effects of contaminated forage with diazinon in the situ procedure or not. The study indicated that effect of diazinon with adding the different levels was significant (p<0.05) for the entire estimated parameters exception “a” fraction and dry matter degradability after 24 h incubation. Also effect of CB on entire estimated parameters for dry matter degradability was insignificant exception dry matter degradability after 48 h incubation. It seems toxicological effect of diazinon on slowly degradable fraction (b) and potentially degradable fraction (a+b) was highest than other estimated parameters and the most decreasing for these parameters was recorded at 5.6 mg of diazinon.

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