This study was designed to examine the percentage y ield and total phenolic contents of various extract s (prepared by using solvents of varying polarity and different extraction methods) of Henna leaves. Total phenolic content in the extracts was determined spectrometrically apply ing the Folin-Ciocalteu assay and calculated as tan nic acid equivalents. Maximum amount of extract (24.83%) was obtained with water, followed by methanol (23.06%) and ethanol (13.25%), while the maximum amount of pheno lics (308.8 µg tannic acid equivalent per gramme of leaves) was obtained with methanol, followed by water (150. 3 µgTAE gdw −1 ) and ethanol (141.5 µgTAE gdw −1 ) using the ultrasound method. Using ultrasound increased the t otal phenolic compounds of the Henna leaves extract. The total phenolic content was not affected by storage in dar k conditions at -18 and 25 °C and in light conditio ns at 25 °C over a period of 10 days, while a significant reduc tion was observed for extracts at 25 °C either in d ark or light conditions after 20 days and for all the extracts after 30 days storage.

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