Title : ( Identification of three species of genus Allium using DNA barcoding )
Authors: Sepideh Anvarkhah , Mohammad Khajeh Hosseini , M0hammad Hassan Rashed Mohassel , Ali Davari Edalat Panah , Hoda Hashemi ,
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Abstract
DNA barcoding is a relatively new method developed to provide rapid, accurate and automatable species identification using standardized DNA sequences as tags. Species identification in this way is usually achieved by the repossession of a short DNA sequence, the “barcode” from a standard part of the genome of a given species. An experiment was conducted to identify three species of genus Allium using five barcode regions including ndhJ, rpoC1, rpoB, YCF5 and rbcL. Results indicated that A. ampeloprasum persicum was the only identified species. Barcode region of YCF5 had the highest SRSP with 93.33%. The highest interspecific diversity and intraspecific divergence with 0.8% and 0.4% were observed in region of ndhJ which was the most sufficient barcode region for species identification.
Keywords
, Allium cepa, Allium ampeloprasum persicum, Allium schoenoprasum L., DNA barcoding, intra specific divergence, SNP
@article{paperid:1035583,
author = {Anvarkhah, Sepideh and Khajeh Hosseini, Mohammad and Rashed Mohassel, M0hammad Hassan and Ali Davari Edalat Panah and Hoda Hashemi},
title = {Identification of three species of genus Allium using DNA barcoding},
journal = {International Journal of Agriculture and Crop Sciences},
year = {2013},
volume = {5},
number = {11},
month = {May},
issn = {2227-670x},
pages = {1195--1203},
numpages = {8},
keywords = {Allium cepa; Allium ampeloprasum persicum; Allium schoenoprasum L.; DNA barcoding;
intra specific divergence; SNP},
}
%0 Journal Article
%T Identification of three species of genus Allium using DNA barcoding
%A Anvarkhah, Sepideh
%A Khajeh Hosseini, Mohammad
%A Rashed Mohassel, M0hammad Hassan
%A Ali Davari Edalat Panah
%A Hoda Hashemi
%J International Journal of Agriculture and Crop Sciences
%@ 2227-670x
%D 2013
