Title : ( Construction of eukaryotic expression vectors encoding CFP-10 and ESAT-6 genes and their potential in lymphocyte proliferation )
Authors: Azam Torabi , Mojtaba Tahmoorespur , Fatemeh Vahedi , Nader Mosavari , Mohammadreza Nassiri ,
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Abstract
Mycobacterium (M.) bovis is the agent of bovine tuberculosis (TB) in a range of animal species, including humans. Recent advances in immunology and the molecular biology of Mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new TB vaccine. The ESAT-6 and CFP-10 proteins of M. bovis are important structural and functional proteins known to be important immunogens. Methods: In the current study, the DNAs encoding these genes were utilized in the construction of pcDNA 3.1+/ESAT-6 and pcDNA3.1+/CFP-10 plasmids. After intramuscular injection of BALB/c mice with these plasmids, ESAT-6 and CFP-10 mRNA expression was assessed by RT-PCR. Mice were inoculated and boosted with the plasmids to evaluate their effects on lymphocyte proliferation. Results: Our results indicate the plasmids are expressed at the RNA level and can induce lymphocyte proliferation. Conclusion: Further study is needed to characterize the effect of these antigens on the immune system and determine whether they are effective vaccine candidates against M. bovis.
Keywords
, Mycobacterium bovis, DNA vaccine, ESAT-6, CFP-10, PPD, Proliferation assay, BALB/c mice
@article{paperid:1036745,
author = {Torabi, Azam and Tahmoorespur, Mojtaba and Fatemeh Vahedi and Nader Mosavari and Nassiri, Mohammadreza},
title = {Construction of eukaryotic expression vectors encoding CFP-10 and ESAT-6 genes and their potential in lymphocyte proliferation},
journal = {Reports of Biochemistry and Molecular Biology},
year = {2013},
volume = {2},
number = {1},
month = {October},
issn = {2322-3480},
pages = {1--7},
numpages = {6},
keywords = {Mycobacterium bovis; DNA vaccine; ESAT-6; CFP-10; PPD; Proliferation assay; BALB/c mice},
}
%0 Journal Article
%T Construction of eukaryotic expression vectors encoding CFP-10 and ESAT-6 genes and their potential in lymphocyte proliferation
%A Torabi, Azam
%A Tahmoorespur, Mojtaba
%A Fatemeh Vahedi
%A Nader Mosavari
%A Nassiri, Mohammadreza
%J Reports of Biochemistry and Molecular Biology
%@ 2322-3480
%D 2013
