The aim of this study is to examine the effect of cost leadership and different strategies on performance of middle-size and large-size manufacturing companies with the intermediation role of organizational learning. This research is a surveydescriptive and practical study. Questionnaire has been used for collecting data. Content and construct validity and Cronbach\'s α method have been used for evaluating the validity and reliability of the questionnaire. The population of this study contains medium-sized and large-sized manufacturing companies of Mashhad industrial town and we have used simple random sampling in this study. PLS software has been used for data analyzing. The analysis of 89 firms confirmed the mediating role of organizational learning between knowledge management and organizational performance. Furthermore, cost leadership strategy has a direct and significant relationship with organizational performance and organizational learning. There are a significant relationship between differentiation strategy and organizational learning and between organizational learning and organizational performance. Moreover, there is not a significant relationship between differentiation strategy and organizational performance

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