Rumen microflora may play an important role in biological degradation of organophosphates and other substances with anti-cholinesterase effect. In this experiment the effects of low-term exposure to the diazinon pesticide (0, 100, 250, 500, 750 and 1000 ppm) with sodium bentonite (0, 1 and 2 % of DM) on the ruminal fermentability of microorganisms was investigated using in vitro batch culture. The correlation coefficient between gas production parameters and other fermentation parameters was also investigated. Results indicated that by increasing diazinon to the culture, there were some toxicological effects in the microorganisms activities which lead to the decrease of potential digestibility of DM and other fermentation parameters such as 96 h cumulative gas production and NH3-N (p<0.05), but the rumen fluid pH was not affected by adding of diazinon. Although sodium bentonite numerically increase the dry matter disappearance rather than control group, however generally it seems rumen fluid couldn’t conceivably be cleaned up the deleterious effects of diazinon at in vitro conditions by sodium bentonite as toxin binder.

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