Gundelia tournefortii L. is a well known plant in mountains of Iran and is found all over the country. It traditionally has some medicinal applications. In this research, Gundelia tournefortiiL. seed was studied as a source of edible oil. Oilwas extracted with immersion method using diethyl ether as a solvent. Acidity, saponification, ester, iodine, peroxide and refractive indices, average molecular weight of fatty acids, unsaponifiable matter, viscosity, color and density of extracted oil were evaluated. In addition, fatty acid composition of oil was determined using gas chromatography analysis. The results showed that seed oil content and saponification value of its oil were 22.8%and 166.05, respectively. Oleic and linoleic acid contents of oil were 27.99%and 54.59%, respectively. It was indicated that the extracted oil is an unsaturated oil and melts at ambient temperature. Beta-sitosterol and stigmasterol were the main unsaponifiable matters of the oil. Color analysis revealed that the predominant color was yellow (0.8 red, 14 yellow). The results indicated that Gundelia tournefortiiL. seed can be potentially applied as an

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