Veterinary Research Forum, ( ISI ), Volume (5), No (2), Year (2014-6) , Pages (107-114)

Title : ( Modeling the growth of Staphylococcus aureus as affected by black zira (Bunium persicum) essential oil, temperature, pH and inoculum levels )

Authors: Abdollah Jamshidi , Saeid Khanzadi , Majid Azizi , Mohammad Azizzadeh , Mohammad Hashem ,

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Abstract

Black zira (Bunium persicum) is a medicinal plant and spice, naturally grows in Iran. The aim of this study was to investigate the combined effects of different concentrations of Bunium persicum essential oil (EO; including 0, 0.08, 0.16 and 0.24%), three incubation temperatures (15, 25 and 35˚C), three levels of pH (5, 6 and 7 adjusted by hydrochloric acid), and three inoculum size (102, 103 and 104 CFU mL-1) on the growth of Staphylococcus aureus in the brain heart infusion broth. Black zira EO was extracted and its component was identified using gas chromatography-mass spectrometry (GC-MS) analyses. The experiment was carried out in triplicate. Growth was monitored by visible turbidity during a 30-day period. To evaluate effects of explanatory variable on time to detection (TTD) of bacterial growth, parametric survival models based on Log-normal distribution was used. All explanatory variables had significant association with time to detection (p < 0.05). The final model accurately predicted the growth initiation and inhibition of S. aureus.

Keywords

, Black zira, Essential oil, predictive modeling, Staphylococcus growth
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@article{paperid:1041920,
author = {Jamshidi, Abdollah and Khanzadi, Saeid and Azizi, Majid and Azizzadeh, Mohammad and Mohammad Hashem},
title = {Modeling the growth of Staphylococcus aureus as affected by black zira (Bunium persicum) essential oil, temperature, pH and inoculum levels},
journal = {Veterinary Research Forum},
year = {2014},
volume = {5},
number = {2},
month = {June},
issn = {2008-8140},
pages = {107--114},
numpages = {7},
keywords = {Black zira; Essential oil; predictive modeling; Staphylococcus growth},
}

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%0 Journal Article
%T Modeling the growth of Staphylococcus aureus as affected by black zira (Bunium persicum) essential oil, temperature, pH and inoculum levels
%A Jamshidi, Abdollah
%A Khanzadi, Saeid
%A Azizi, Majid
%A Azizzadeh, Mohammad
%A Mohammad Hashem
%J Veterinary Research Forum
%@ 2008-8140
%D 2014

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