Title : ( Impact of different culture media on hairy roots growth of Valeriana officinalis L. )
Authors: Ali Pakdin , Mohammad Farsi , Ghorban Ali Nematzadeh , Amin Mirshamsi kakhki ,
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Abstract
Transformed hairy root cultures of Valeriana officinalis were established by infection with Agrobacterium rhizogenes strain ATCC 15834. To determine the effect of different media on the growth of V. officinalis hairy roots, MS, B5 media (1.0X and 0.5X strength), N6 medium and a modified MS medium without phytohormones were used. In addition, different NH4+ to NO3- ratios in MS medium were studied. The effects of these treatments were evaluated after 21 days of culture in relation to hairy root growth. B5 and ½ B5 media were the best basal media for hairy root growth. MS medium supplemented with a 20:20 ratio (mM) of NH4+ to NO3- displayed highest growth rates and biomass yield in hairy root cultures. The present study demonstrated that the composition of culture medium and the ratio of different nitrogen sources have significant impact on the growth of V. officinalis hairy roots.
Keywords
, Valeriana officinalis, hairy root, medium composition
@article{paperid:1045210,
author = {Pakdin, Ali and Farsi, Mohammad and Ghorban Ali Nematzadeh and Mirshamsi Kakhki, Amin},
title = {Impact of different culture media on hairy roots growth of Valeriana officinalis L.},
journal = {Acta Agriculturae Slovenica},
year = {2014},
volume = {103},
number = {2},
month = {December},
issn = {1581-9175},
pages = {299--305},
numpages = {6},
keywords = {Valeriana officinalis; hairy root; medium composition},
}
%0 Journal Article
%T Impact of different culture media on hairy roots growth of Valeriana officinalis L.
%A Pakdin, Ali
%A Farsi, Mohammad
%A Ghorban Ali Nematzadeh
%A Mirshamsi Kakhki, Amin
%J Acta Agriculturae Slovenica
%@ 1581-9175
%D 2014
