Objective. Our point is to determine if the treatment of the 3rd degree burn makes any difference if sprayed cultured autologus keratinocyte used alone or in combination with Fibrin glue. Design. Experimental in-vivo study.Animals. 30 male Wistar rats.Procedures. Thirty male Wistar rats were selected and their keratinocytes were isolated and cultured from small skin biopsy. Rats were divided randomly into 3 equal groups and three full-thickness round burn wounds were created on their back. Wounds were treated with normal saline (control group), AKS (test group1) and AKS + Fibrin (AKS+ F) (test group2) in each rat.The wounds were photographed on selected days (0, 3, 5, 7, 10 and 14) and the percentage of wounds contraction was calculated with image analyzer. Biopsy samples were taken as well for histological studies. Results. The results showed faster wound contraction for AKS and AKS+ F groups during 14 days period than control group (P<0.05(. Histological observations showed significant difference in inflammation and fibrotic tissue formation between groups but other parameters did not show any remarkable difference. Conclusion and Clinical Relevance. It can be concluded that fibrin glue could prevent cells from dripping out of the wound and also speed up the wound contraction and extend the inflammation and fibrotic tissue formation but it did not have any effect on fastening the re-epithelialisation and granulation tissue formation during 14 days.

Keywords