Title : ( EXPRESSION OF FOOT AND MOUTH DISEASE VIRUS (FMDV) CAPSID PROTEIN VP1 IN CHLAMYDOMONAS REINHARDTII AS A POSSIBLE SOURCE OF RECOMBINANT VACCINE )
Authors: maziar habibi , Saeid Malekzadeh Shafaroudi , Hassan Marashi , Nasrin Moshtaghi , Mohammadreza Nassiri , Afsane Mohkami , Saeid Zibaee ,
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Abstract
A chimeric construct of foot and mouth disease virus capsid protein VP1 was expressed in Chlamydomonas reinhardtii via Agrobacterium -mediated genetic transformation. In tegration of the foreign gene in nuclear genome of the algae was confirmed by PCR and expression of the recombinant protein was confirmed at translational level. Results showed that the foreign gene was integrated in nucleus and the recombinant protein was produced in the microalgal cells. Transformation process together with detection of recombinant protein are discussed.
Keywords
, genetic transformation, Foot and Mouth Disease, chlamydomonas, , recombinant vaccine, VP1
@article{paperid:1045763,
author = {Habibi, Maziar and Malekzadeh Shafaroudi, Saeid and Marashi, Hassan and Moshtaghi, Nasrin and Nassiri, Mohammadreza and Mohkami, Afsane and Saeid Zibaee},
title = {EXPRESSION OF FOOT AND MOUTH DISEASE VIRUS (FMDV) CAPSID PROTEIN VP1 IN CHLAMYDOMONAS REINHARDTII AS A POSSIBLE SOURCE OF RECOMBINANT VACCINE},
journal = {International Journal of Plant, Animal and Environmental Sciences},
year = {2014},
volume = {4},
number = {2},
month = {January},
issn = {2231-4490},
pages = {644--648},
numpages = {4},
keywords = {genetic transformation; Foot and Mouth Disease; chlamydomonas، ; recombinant vaccine; VP1},
}
%0 Journal Article
%T EXPRESSION OF FOOT AND MOUTH DISEASE VIRUS (FMDV) CAPSID PROTEIN VP1 IN CHLAMYDOMONAS REINHARDTII AS A POSSIBLE SOURCE OF RECOMBINANT VACCINE
%A Habibi, Maziar
%A Malekzadeh Shafaroudi, Saeid
%A Marashi, Hassan
%A Moshtaghi, Nasrin
%A Nassiri, Mohammadreza
%A Mohkami, Afsane
%A Saeid Zibaee
%J International Journal of Plant, Animal and Environmental Sciences
%@ 2231-4490
%D 2014
