The aim of this study is to investigate the in vitro proliferation of Astragalus adscendens. Explants were taken from hypocotyl and cotyledon, then they were cultured on the basic medium of Murashige and Skoog (MS) that has been complemented with various plant growth regulators, (NAA, BAP, Kinetin, Zeatin). Callus induction was significantly affected by different concentrations of PGRs. Callus formation was observed from hypocotyl explants, but they were not induced to adventitious shoot regeneration and most of them were turned into brown. Therefore, rapid multiplication, performed using shoot apical buds, and obtained from 15 days old sterile seedlings. Apical buds were cultured on MS medium containing various levels of BAP, Kinetin and Zeatin (0.5, 1.0, 2.0 and 4.0 mg/l) alone or in combination with 0.5 mg/l NAA. The highest number of shoot regenerants (8.5/explants) and leaves (22.4/explants) obtained on MS medium with 4 mg/l BAP. Rooted plantlets were successfully acclimatized in pots with 1:1:1 mixture of soil, peat and perlite.

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