tBene is an edible fruit from the tree Pistacia atlantica subsp. mutica, and is of steadily growing interestin recent years due to its significant antioxidant properties and potential health benefits. An antioxi-dant activity-guided fractionation of the methanol extract from Bene hull together with an integratedapproach of HPLC–DAD, LC–MS and1H NMR techniques led to the identification of main antioxidantphenolic compounds for the first time. Radical scavenging activity of each fraction/compound wastested using DPPH and FRAP assays. The phenolic content of the fractions was also determined byFolin–Ciocalteu’s method. The main identified antioxidant compounds were luteolin (46.53% w/w of totalextract), gallic acid (9.84% w/w), 2-O-galloylisoquercitrin (0.53% w/w), quercetin 3-rutinoside (0.34%w/w) and 2-O-cis-caffeoylquercitrin (0.26% w/w). The minor antioxidant compounds were also iden-tified by liquid chromatography–positive/negative electrospray ionization tandem mass spectrometry.The structure–antioxidant activity relationship of identified phenolics are also discussed in this paper.

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