Understanding the interactions and relationships between fertilizer and minerals help to have optimum fertilization practice with the lowest costs. In a split-split plot field experiment, we studied the effect of some organic substrate (control (soil), spent mushroom substrate (SMS), cow manure and vermicomposts), different levels of phosphorus (at 0, 75, 100 and 125 kg h -1 ) and zinc foliar application (0 and 1000 mgl-1 ) to investigate the best treatment for some characters of medicinal pumpkin (Cucurbita pepo L.) such as: leaf, stem length, fruit weight, dry matter percentage, seed number, phosphorus and protein content of seeds and TSS. For lonely application of organic substrates, phosphorus concentration and zinc foliar application; vermicomposts, 125 P (kg.ha-1 ) and 1000 Zn (mg l-1 ) treatments resulted the high improved in pumpkin seeds and qualities. For combining application of organic substrate×P levels, organic substrate×Zn levels, P levels× Zn levels and organic substrate×P levels×Zn levels, reslts showed the maximum of seed number with high quality (protein) were found in cow manure×125P, vermicomposts×1000 Zn, 125P×1000Zn and vermicomposts×100P×1000 Zn, respectively.

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