International Journal of Fertility and Sterility, ( ISI ), Volume (10), No (2), Year (2016-7) , Pages (253-260)

Title : ( Zeta Sperm Selection Improves Pregnancy Rate and Alters Sex Ratio in Male Factor Infertility Patients: A Double-Blind, Randomized Clinical Trial )

Authors: Mohammad Hossein Nasr Esfahani , Mohammad Reza Deemeh , Marziyeh Tavalaee , Mohammad Hadi Sekhavati , Hamid Gourabi ,

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Abstract

Background: Selection of sperm for intra-cytoplasmic sperm injection (ICSI) is usually considered as the ultimate technique to alleviate male-factor infertility. In routine ICSI, selection is based on morphology and viability which does not necessarily preclude the chance injection of DNA-damaged or apoptotic sperm into the oocyte. Sperm with high negative surface electrical charge, named “Zeta potential”, are mature and more likely to have intact chromatin. In addition, X-bearing spermatozoa carry more negative charge. Therefore, we aimed to compare the clinical outcomes of Zeta procedure with routine sperm selection in infertile men candidate for ICSI. Materials and Methods: From a total of 203 ICSI cycles studied, 101 cycles were allocated to density gradient centrifugation (DGC)/Zeta group and the remaining 102 were included in the DGC group in this prospective study. Clinical outcomes were compared between the two groups. The ratios of X- and Y bearing sperm were assessed by fluorescence in situ hybridization (FISH) and quantitative polymerase chain reaction (qPCR) methods in 17 independent semen samples. Results: In the present double-blind randomized clinical trial, a significant increase in top quality embryos and pregnancy rate were observed in DGC/Zeta group compared to DGC group. Moreover, sex ratio (XY/XX) at birth significantly was lower in the DGC/Zeta group compared to DGC group despite similar ratio of X/Y bearings spermatozoa following Zeta selection. Conclusion: Zeta method not only improves the percentage of top embryo quality and pregnancy outcome but also alters the sex ratio compared to the conventional DGC method, despite no significant change in the ratio of X- and Y- bearing sperm population (Registration number: IRCT201108047223N1

Keywords

, Zeta Potential, Density Gradient Centrifugation, Sex Ratio, Embryo Quality, Pregnancy
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@article{paperid:1059036,
author = {Mohammad Hossein Nasr Esfahani and Mohammad Reza Deemeh and Marziyeh Tavalaee and Sekhavati, Mohammad Hadi and Hamid Gourabi},
title = {Zeta Sperm Selection Improves Pregnancy Rate and Alters Sex Ratio in Male Factor Infertility Patients: A Double-Blind, Randomized Clinical Trial},
journal = {International Journal of Fertility and Sterility},
year = {2016},
volume = {10},
number = {2},
month = {July},
issn = {2008-076X},
pages = {253--260},
numpages = {7},
keywords = {Zeta Potential; Density Gradient Centrifugation; Sex Ratio; Embryo Quality; Pregnancy},
}

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%0 Journal Article
%T Zeta Sperm Selection Improves Pregnancy Rate and Alters Sex Ratio in Male Factor Infertility Patients: A Double-Blind, Randomized Clinical Trial
%A Mohammad Hossein Nasr Esfahani
%A Mohammad Reza Deemeh
%A Marziyeh Tavalaee
%A Sekhavati, Mohammad Hadi
%A Hamid Gourabi
%J International Journal of Fertility and Sterility
%@ 2008-076X
%D 2016

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