This experiment was conducted to determine the apparent protein digestibility, metabolizable energy (AME) and metabolizable energy correction of zero nitrotion (AMEn) of poultry by-product meal (PBPM) from two industrial poultry slaughter houses on Ross 308 male broiler chickens. The experiment consisted of seven dietary treatments and three replicates per treatment with three broiler chickens male per replicate in a compeletely randomized design. Dietary treatments consisted of a control cornsoybean diet, and levels 3, 6 and 9% PBPM produced by slaughter house 1 and levels 3, 6 and 9% PBPM produced by slaughter house 2. Chromic oxide was added to the experimental diets as indigestible marker. Apparent protein digestibility, AME and AMEn of each diets were determined with two methods of sample collection of ileum and excreta in 21-28d of age. Use of PBPM had no significant effect on performance of broiler chicks during period of digestibility experiments (P>0.05). Results indicated that there were significant differences in apparent protein digestibility, AME and AMEn in PBPM groups vs control group by excreta sampling procedure (P<0.05). The apparent protein digestibility, AME and AMEn based on ileum sampling procedure significantly decreased in level 9%PBPM slaughter house 2 (P<0.05). The site of measurement had no significant effect on protein digestibility AME and AMEn among experimental treatments (P>0.05), but ileal protein digestibility was significantly greater than excreta as concern the two sampling methods (P<0.05).

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