Secondary lymphoid-tissue chemokine (SLC/CCL21) is a CC chemokine that is basically expressed in different lymphoid tissues and binds to chemokine receptor CCR7 on active dendritic cells (DCs) and increase T-cell activities. Two methods of expressing recombinant proteins in plants exist; transient expression by Agroinfiltrationand stable transformation of plant genome.each having advantages depending on the goal of study. Transient transformation of tobacco leaf is a fast technique for assessing gene before generating stable plant lines using a more time-consuming technique. Transient expression takes from 3to 7 days whereas transgenic linesare generated after 2-4 months. Soagroinfiltration as a rapid method for production presence of recombinant antigen of C-C chemokine ligand 21 (CCL21) intobacco leaves.Foreign gene expression was evaluated using semi quantitative RT-PCR, Real time PCR , protein dot blot and enzyme-linked immunosorbent assay (ELISA) and western blot,All results show that the foreign gene was transcribed in transformed leaves. Finally, the efficacy of agroinfiltration for transient expression of CCL21 recombinant protein in tobacco was illustrated in this study.

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