Micro-organisms, tissues, cells, organelles, enzymes, peptides and DNA are used to identify the target substance called as specific or non-specific biosensor. Due to the importance of flavonoids as antioxidants and expensive and time consuming methods, detection of flavonoids via biosensor is growing. Plant bionanosensor is a new approach and certain type of biosensor that converts the concentration of flavonoids into visible rotation and flavonoids are detected by different rotations of concentration. The rotations of crataegus oxyacantha -Hawthorn- extract- flavonoids -with plant bionanosensor were analyzed in a completely randomized design with three replications by sas9.1 software. Bionanosensor is capable to detect 0.2025-0.02 mg, 0.0125-0.01 mg, 0.00625-0.005 mg and 0 mg flavonoid in 1.100 cc -0 mg flavonoid as a control-.The different levels of flavonoid rotation were significant with a probability of 99%. So far, no report was made on plant biosensor.

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