Title : ( Purification of ISS protein from avian pathogenic Escherichia coli strain χ1378 by affinity chromatography )
Authors: B. Nayeri , A. Derakhshandeh , T. Zahraei Salehi , S. Gholami , Elaheh Ebrahimzadeh Abkooh , Z. Naziri ,
دانلود فایل برای اعضای دانشگاهAccess to full-text not allowed by authors
Abstract
Colibacillosis due to avian pathogenic Escherichia coli affects poultry worldwide. A major virulence factor is increased serum survival (ISS) gene resistant to the complement system. ISS protein of virulent E coli from avian Colibacillosis was expressed and purified using gene specific primers to generate a 359 bp product. ISS protein was analyzed by SDS-PAGE, affinity chromatography and Xa factor for purification and separation of the recombinant protein. SDS-PAGE and western blot analyzes confirmed purity and antigenicity of the protein showing a 8 kDa band protein.
Keywords
, Purification, ISS protein, Escherichia coli, affinity chromatography
@article{paperid:1066749,
author = {B. Nayeri and A. Derakhshandeh and T. Zahraei Salehi and S. Gholami and Ebrahimzadeh Abkooh, Elaheh and Z. Naziri},
title = {Purification of ISS protein from avian pathogenic Escherichia coli strain χ1378 by affinity chromatography},
journal = {Online Journal of Veterinary Research},
year = {2014},
volume = {18},
number = {5},
month = {May},
issn = {1328-925X},
pages = {391--397},
numpages = {6},
keywords = {Purification; ISS protein; Escherichia coli; affinity chromatography},
}
%0 Journal Article
%T Purification of ISS protein from avian pathogenic Escherichia coli strain χ1378 by affinity chromatography
%A B. Nayeri
%A A. Derakhshandeh
%A T. Zahraei Salehi
%A S. Gholami
%A Ebrahimzadeh Abkooh, Elaheh
%A Z. Naziri
%J Online Journal of Veterinary Research
%@ 1328-925X
%D 2014
