Gene, Cell and Tissue, Volume (3), No (3), Year (2016-7) , Pages (1-7)

Title : ( Inducing the effects of acellular dermal matrix on blastema tissue originated from the pinna of New Zealand white rabbit, in vitro )

Authors: tayebeh dahmardeh ghalenow , Nasser Mahdavi SHahri , Maryam Moghaddam Matin , Morteza Behnam Rassouli , Roya Lari ,

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Abstract

Background: Extracellular matrix (ECM) functions as a scaffold for tissue morphogenesis and regeneration, promotes the mainte-nance of differentiated tissue and plays an extremely indispensable role in cell proliferation and differentiation. As a corollary ofthis importance, studying the cell behaviors without considering the ECM is to some extent impossible. Deletion of cells from ECMobtains natural three dimensional matrices that ape the in vivo functions of ECM.Objectives: The current study aimed to verify whether acellular dermal matrix (ADM) is a suitable 3D-matrix for blastema tissuecells originated from pinna of rabbit and study different behaviors of these cells on ADM.Materials and Methods: To prepare ADM, small pieces of skin were decellularized by repeated snap freeze-thaw and treatment withsodium dodecyl sulfate as a detergent. Decellularization was verified and proved by histological tests. Blastema rings were preparedby double punching the pinnas of male New Zealand White rabbits and cultured in vitro after assembling with ADM. Specimens werestudied after 5, 10, 15, 20 and 25 days of culturing.Results: Migration of cells started on the fifth day. As a result of this migration, most cells were located on the surface of the scaffoldand formed epiderm-like structure and some penetrated into the scaffold. By day 25, cells which had penetrated into the scaffoldhad various destinies such as becoming elongated spindle-shaped cells and creating blood vessel-like structures.Conclusions: ADM can induce migration and probably differentiation of blastema tissue cells; therefore, it is a suitable 3D-modelto study cell behaviors in vitro.

Keywords

, Acellular Dermal Matrix, Blastema Tissue, Extracellular Matrix, Decellularization
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@article{paperid:1066930,
author = {Dahmardeh Ghalenow, Tayebeh and Mahdavi SHahri, Nasser and Moghaddam Matin, Maryam and Behnam Rassouli, Morteza and Lari, Roya},
title = {Inducing the effects of acellular dermal matrix on blastema tissue originated from the pinna of New Zealand white rabbit, in vitro},
journal = {Gene, Cell and Tissue},
year = {2016},
volume = {3},
number = {3},
month = {July},
issn = {2345-6833},
pages = {1--7},
numpages = {6},
keywords = {Acellular Dermal Matrix; Blastema Tissue; Extracellular Matrix; Decellularization},
}

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%0 Journal Article
%T Inducing the effects of acellular dermal matrix on blastema tissue originated from the pinna of New Zealand white rabbit, in vitro
%A Dahmardeh Ghalenow, Tayebeh
%A Mahdavi SHahri, Nasser
%A Moghaddam Matin, Maryam
%A Behnam Rassouli, Morteza
%A Lari, Roya
%J Gene, Cell and Tissue
%@ 2345-6833
%D 2016

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