Frontiers in Microbiology, Volume (9), No (1578), Year (2018-7) , Pages (1-9)

Title : ( Construction and Characterization of a New Recombinant Vector to Remove Sulfate Repression of dsz Promoter Transcription in Biodesulfurization of Dibenzothiophene )

Authors: Somayye Khosravinia , Mahmood Akhavan Mahdavi , Reza Gheshlaghi , Hesam Dehghani , B. Rasekh ,

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Abstract

Biodesulfurization (BDS) is an environmentally friendly desulfurizing process with the potential of replacing or adding to the current expensive technologies for sulfur removal from fossil fuels. The BDS, however, still suffers from low biocatalyst activity. One reason is repression of dsz promoter transcription in presence of inorganic sulfate that impedes translation of Dsz enzymes required for desulfurization pathway. One approach to solve this problem is replacing the native promoter with a new promoter that is no longer repressed. In this study, dsz genes from desulfurizing strain Rhodococcus sp. FUM94 was cloned in an alkane responsive promoter, pCom8, and expressed in Escherichia coli BL21 (DE3) as a host. The recombinant was not susceptible to inorganic sulfate in the culture medium. Desulfurizing activity of recombinant strain versus wild type indicated that in a sulfate containing medium, BDS yield of recombinant increased from 16.0%  0.9 to 34.0%  1.9% when dibenzothiophene (DBT) concentration (dissolved in ethanol) increased from 25 to 100 ppm. Also, 2-hydroxy biphenyl (2-HBP) production rate improved 8.5-fold (from 0.302  0.020 to 2.57  0.14 mmol 2-HBP (kg DCW)

Keywords

, biodesulfurization, biocatalyst, transcription, dsz promoter, sulfate repression, recombinant vectorpCom8
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@article{paperid:1069435,
author = {Khosravinia, Somayye and Akhavan Mahdavi, Mahmood and Gheshlaghi, Reza and Dehghani, Hesam and B. Rasekh},
title = {Construction and Characterization of a New Recombinant Vector to Remove Sulfate Repression of dsz Promoter Transcription in Biodesulfurization of Dibenzothiophene},
journal = {Frontiers in Microbiology},
year = {2018},
volume = {9},
number = {1578},
month = {July},
issn = {1664-302X},
pages = {1--9},
numpages = {8},
keywords = {biodesulfurization; biocatalyst; transcription; dsz promoter; sulfate repression; recombinant vectorpCom8},
}

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%0 Journal Article
%T Construction and Characterization of a New Recombinant Vector to Remove Sulfate Repression of dsz Promoter Transcription in Biodesulfurization of Dibenzothiophene
%A Khosravinia, Somayye
%A Akhavan Mahdavi, Mahmood
%A Gheshlaghi, Reza
%A Dehghani, Hesam
%A B. Rasekh
%J Frontiers in Microbiology
%@ 1664-302X
%D 2018

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