Title : ( Genotyping of human cystic echinococcosis in Northeastern Iran )
Authors: Hassan Borji , Mostafa Mehrabi Bahar , Abolghasem Naghibi , Seyed Hossein Fattahi ,
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Abstract
Cystic echinococcosis (CE) is a human public health problem in Northeast of Iran and there is limited information regarding the genotypes of Echinococcus granulosus senso lato (s.l.) in human in this region. In the present study, we determined the genotypes of E. granulosus s.l. infecting human in northeastern Iran from Khorasan Razavi provine using PCR-RFLP of rDNA ITS1 region. From April 2013 to December 2016, 50 hepatic hydatid cysts were recovered from 50 patients who underwent surgical procedure. Protoscoleces were collected from individual cysts, DNA was extracted, and the rDNA ITS1 gene was amplified by PCR and genotypes confirmed by RFLP. All the cyst materials were identified as Echinococcus granulosus senso stricto (s.s.), indicating this species as the main cause of CE in humans in northeast of Iran.
Keywords
, human, cystic echinococcosis, genotype, PCR-RFLP
@article{paperid:1072039,
author = {Borji, Hassan and Mostafa Mehrabi Bahar and Naghibi, Abolghasem and Seyed Hossein Fattahi},
title = {Genotyping of human cystic echinococcosis in Northeastern Iran},
journal = {Annals of Parasitology},
year = {2018},
volume = {64},
number = {4},
month = {December},
issn = {2299-0631},
pages = {323--329},
numpages = {6},
keywords = {human; cystic echinococcosis; genotype; PCR-RFLP},
}
%0 Journal Article
%T Genotyping of human cystic echinococcosis in Northeastern Iran
%A Borji, Hassan
%A Mostafa Mehrabi Bahar
%A Naghibi, Abolghasem
%A Seyed Hossein Fattahi
%J Annals of Parasitology
%@ 2299-0631
%D 2018
