Title : ( Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS )
Authors: Ameneh Tarakemeh , Majid Azizi , Vahid Rowshan , Hassan Salehi , Rosella Spina , Francois Dupire , Hossein Arouiee , Dominique Laurain-Mattar ,
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Abstract
Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a common species distributed in Iran. In this study, calli, roots and bulblets were regenerated from N. tazetta var. Meskin. In order to report alkaloid profile and quantify galanthamine and lycorine, methanolic extract of different in vitro tissues were subjected to alkaloid purification by SPE (solid phase extraction) method, followed by GC-MS analysis. In general, galanthamine and lycorine were detected in all in vitro tissues while demethylmaritidine was only identified in bulblets. Bulblet was identified as the best source of main Amaryllidaceae alkaloids regarding the highest quantities of galanthamine and lycorine with 15 and 731 µg/g dry weight (DW) respectively.
Keywords
, Amaryllidaceae alkaloids, Galanthamine, Lycorine, Narcissus, Tissue culture.
@article{paperid:1076082,
author = {Tarakemeh, Ameneh and Azizi, Majid and وحید روشن and حسن صالحی and Rosella Spina and Francois Dupire and Arouiee, Hossein and Dominique Laurain-Mattar},
title = {Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS},
journal = {International Journal of Horticultural Science and Technology},
year = {2019},
volume = {6},
number = {2},
month = {December},
issn = {2322-1461},
pages = {151--157},
numpages = {6},
keywords = {Amaryllidaceae alkaloids; Galanthamine; Lycorine; Narcissus; Tissue culture.},
}
%0 Journal Article
%T Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS
%A Tarakemeh, Ameneh
%A Azizi, Majid
%A وحید روشن
%A حسن صالحی
%A Rosella Spina
%A Francois Dupire
%A Arouiee, Hossein
%A Dominique Laurain-Mattar
%J International Journal of Horticultural Science and Technology
%@ 2322-1461
%D 2019
