Treated fish wastes have found many applications in industry and medicine. Besides, nowadays low-cost scaffold with antimicrobial activity which can accelerates the process of wound healing is very demanding. In this study fish swim bladder (FSB), taken from Rutilus frisii , which is a disposable waste was doped with silver nanoparticles (AgNPs) and evaluated as antimicrobial wound dressing. The scanning electron microscopy (SEM) micrographs showed the presence of AgNPs on the scaffold. Histological observation confirmed cells and muscle removal from FSB and collagen preservation. There was significant antibacterial activity even in 50 ppm AgNPs concentration against pathogenic bacteria, swelling ratio was rather low, and cytotoxic assay revealed that the AgNPs-FSB scaffold had no toxic effect on human foreskin fibroblast (HFF) cells. Interestingly, despite the porous structure, the AgNPs-FSB scaffold was found to be a suitable barrier to microbial penetration even after 72 h. Further study showed the gradual release of AgNPs during 24 h. In conclusion, biofabricated FSB prepared in this study have appropriate characteristics notably encompassing a high quantity of collagen and broad-spectrum antimicrobial activity. Also, its porous structure made it suitable as a 3-D structure for the growth of cells and adding other antimicrobial nano-sized materials.

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