Silver nanoparticles are widelyusedin manufacturing of different productsconsidering their unique physical and chemical properties. Also they have been noticed in medical diagnosis and treatment because of their antibacterial properties. Physical and chemical methods of producing nanoparticles, are expensive and are not safe enough as toxic substances may remain in the final preparations. To solve this problem, biological production of nanoparticles is considered as an efficient alternative method. In present study, synthesis of silver nanoparticles via seeds, petals, roots,and hairy root extracts of Calendula officinaliswere performed. These nanoparticles were characterized by means of spectrophotometer, particle size analyzer and transmission electron microscope. Nanoparticles which were synthesized by hairy root extract showed the highest absorption at430 nm (1/6 a.u) and the smallest size (5/3 nm), incomparisonto otherexaminedparticles. Results confirmed the better performance of hairy root extractsin the synthesis of silver nanoparticles.

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