Pseudomona aeruginosa could cause serious infections in hospitals and is highlighted as a source of financial problems in farms. The revelation of drug resistant, particularly multi-drug resistant (MDR)P. aeruginosa is important around the world. The efflux pump activity is identified as one of the most important intrinsic resistant mechanisms in P. aeruginosa. A total of 96 P. aeruginosa isolates from inpatient and animal sources were tested for antimicrobial susceptibility and subjected to multiplex PCR (mPCR) assay to detect MexAB-OprM efflux pump system. The human isolates have shown the highest resistance against cefazolin, ampicillin, nalidixic acid, trimethoprim/sulfamethoxazole, cepha-lothin, oxacillin (100%). All farm animal isolates were resistant to cefazolin, kanamycin, amoxicillin clavulanic acid, and cephalothin (100%). In both isolate groups, the presence of MexA was more common than that of MexB.MexAB-OprM was demonstrated as a valuable mechanism in P. aerugi-nosa antimicrobial resistant strains.

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