Title : ( Optimization of gamma‐aminobutyric acid production by Lactobacillus brevis PML1 in dairy sludge‐based culture medium through response surface methodology )
Authors: Fereshteh Falah , Alireza Vasiee , Behrooz Alizadeh Behbahani , Farideh Tabatabaei yazdi , Sayed Ali Mortazavi ,
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Abstract
Gamma-aminobutyric acid (GABA) is a pharmaceutical, bioactive amino acid that can produce by some species of Lactic Acid Bacteria (LAB). For the first time, we evaluated the production of GABA by Lactobacillus brevis PML1 in the medium that contain the contaminant food bio-product like dairy sludge and soybean meal. GABA production was analyzed by chromatography (TLC, HPLC) and the features of fermented extract which contains this amino acid were evaluated. The results of Response Surface Methodology (RSM) of Central Composite Design (CCD) at p < .05 showed 300 ppm of GABA production in optimal treatment including 14.77% dairy sludge powder, 6.27% soybean meal, and 0.49% ammonium sulfate (32°C for 120 hr fermentation). The results of fermented extract also showed the acceptable antimicrobial, antioxidant, and toxicity (against cancer cell) properties. Also, L. brevis PML1has not shown any hemolytic or DNase activity which confirm its safety aspects. According to the results, this new culture can be used as a cheap substrate to biological production of GABA, by L. brevis PML1 in various food and pharmaceutical formulations.
Keywords
, dairy sludge, food waste, GABA, HPLC, Lactobacillus brevis
@article{paperid:1084817,
author = {Falah, Fereshteh and Vasiee, Alireza and Behrooz Alizadeh Behbahani and Tabatabaei Yazdi, Farideh and Mortazavi, Sayed Ali},
title = {Optimization of gamma‐aminobutyric acid production by Lactobacillus brevis PML1 in dairy sludge‐based culture medium through response surface methodology},
journal = {Food Science and Nutrition},
year = {2021},
volume = {9},
number = {6},
month = {May},
issn = {2048-7177},
pages = {3317--3326},
numpages = {9},
keywords = {dairy sludge; food waste; GABA; HPLC; Lactobacillus brevis},
}
%0 Journal Article
%T Optimization of gamma‐aminobutyric acid production by Lactobacillus brevis PML1 in dairy sludge‐based culture medium through response surface methodology
%A Falah, Fereshteh
%A Vasiee, Alireza
%A Behrooz Alizadeh Behbahani
%A Tabatabaei Yazdi, Farideh
%A Mortazavi, Sayed Ali
%J Food Science and Nutrition
%@ 2048-7177
%D 2021
