Abstract Background The aim of current study was to determine the protection e????cacy of Eucalyptus globulus and Syzygium aromaticum essential oils nanoemulsions-loaded textiles versus bulk essential oil- treated textiles against the malaria vector, Anopheles stephensi. Methods The components of E. globulus and S. aromaticum essential oils were determined using gas chromatography/mass spectrometry. Then, the nanoemulsions of both essential oils were prepared using a low energy emulsi????cation method. Their stability and droplet sizes were determined, and the repellent e????cacy against landings/bites of the starve mosquito females was examined using textile panels of polyester/cotton, impregnated with serial concentrations of the nano-emulsion. Results The main compositions of E. globulus essential oil were 1, 8-cineol (64.58%) and alpha-pinene (10.63%), whereas those of S. aromaticum essential oil were 2-methoxy-3-(2-propenyl) (77.04%) and transcaryophyllene (11.99%). Transparent oil in water nanoemulsion system consisting of essential oils, Tween-20, Tween-80 and propylene glycol was developed. The median droplet size was 11.2-23.1nm depending on dilution ratio. Protection time of nanoemulsion-loaded textile (285 ± 30 min) was noticeably higher than that of bulk essential oils (< 5 min). Conclusions It was concluded that nanoemulsion of essential oils may be interesting options in control of mosquitorelated diseases.

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