In this study, 32 adult male mice were divided into four groups (8 in each group), control (received normal saline) and the three experimental groups (received Crocin 4, 20,100 mg/kg every day) for 6 weeks. At the end of the experiment, blood testosterone level was analyzed by enzyme immunoassay and sperm parameters (count, motility, and viability) were evaluated. Data were analyzed by one-way ANOVA, using SPSS software. After administration of Crocin at a dosage of 100 mg/ kg, the results clearly showed that the number of sperm was significantly reduced compared to the control. In experimental groups (20 and 100 mg/kg), a significant increase in testosterone levels in blood serum compared to the control group was shown. But, in the Crocin group (4 mg/kg), no significant difference in testosterone levels than the control group was shown. Compared with the control group, the average testosterone level of the Crocin group (20 mg/kg) was significantly increased. In conclusion, higher doses (100 mg/kg) of Crocin administration may negatively affect the reproductive system by reducing sperm count, sperm motility, and testicular damage. However, a relatively safe dose of Crocin (20 mg/kg) is required in clinical trials.

Keywords