The use of treated wastewater is one of the ways to resolve a lack of water concerns. In this study, different responses of two kinds of grasses (C3: Lolium perenne L. and C4: Cynodon dactylon L.) and four levels of wastewater (control, 25, 50, 75, and 100%) were evaluated at morphological and physiological levels. The concentration of Cd, Cr, Ni, As, and Cu elements significantly increased in leaf tissue under the toxic levels of wastewater (100%). The results showed that Pn, Tr, gs, WUEi, and chlorophyll significantly increased when grasses were under 50% wastewater treatment. Plantsʼ growth parameters including root, and shoot dry weights exhibited a significant increase under low concentration of wastewater while photosynthetic index and growth parameters showed a high reduction under high wastewater concentration. The results showed that by the handling of wastewater and the utilize of suitable concentrations, this unusual water source can be used for irrigation of grasses that need high water demands such as Lolium perenne and Cynodon dactylon.

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